Product Name
BiP | Lumenal-binding protein, ALP-conjugated (40 µg)
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540 Q9LKR3, BiP2 At5g42020 F4K007 , BiP3 At1g09080 Q8H1B3
Background
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
Purity
Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP.
Reactivity
A. thaliana, B. napus, C. sativus, R. sativa L. Tokinashi-daikon, S. oleracea, S. lycopersicum, S. tuberosum, T. aestivum, Z. mays, O. europaea, P. abies, P. patens, Ch. reinhardtii
Confirmed reactivity
Arabidopsis thaliana, Brassica napus, Chlamydomonas reinhardtiiCucumis sativus, Nicotiana benthamiana, Raphanus sativa L. Tokinashi-daikon, Olea europaea, Picea abies, Physcomitrium patens, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Zeay mays
Predicted reactivity
Arabis alpina, Capsella rubella, Capsicum annuum, Citrus clementina, Citrus sinsensis, Eucalyptus grandis, Glycine max, Hordeum vulgare, Isatis tincorina, Nicotiana tabacum, Oryza sativa, Prunus persica, Triticum aestivium. Picea sitcHensis, Populus trichocarpa, Ricinus comminus, Vitis vinifera
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Expected/apparent MW
73.5 | 80 kDa
Tested applications
ELISA (ELISA), Western blot (WB)
Recommended dilution
1 : 8000 (ELISA), 1 : 2000 (WB)
Storage
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Datasheet 