Product Name
aadA1 | Aminoglycoside adenyltransferase (chloroplast transformation marker)
Immunogen
KLH-conjugated peptide derived from known aadA1 protein sequences
Background
aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection.
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Reactivity
Nicotiana tabacum, Chlamydomonas reinhardtii
Confirmed reactivity
aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii
Predicted reactivity
Acinetobacter baumannii, Enterobacter agglomerans, Klebsiella pneumoniae, Klebsiella oxytoca, Kluyvera ascorbata, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Worthington, Staphylococcus aureus, Serratia sp., Shigella flexneri
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
30 | 30 kDa
Tested applications
Western blot (WB)
Recommended dilution
1 : 3 000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 