Product Name
BAR | Phosphinothricin N-acetyltransferase (170-183)
Immunogen
KLH-conjugated peptide derived from position 170-183 of Phosphinothricin N-acetyltransferase (BAR or BASTA), UniProt: P16426
Background
Phosphinothricin N-acetyltransferase (BAR) is an enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance, herbicide resitance gene. BAR (BASTA) gene is used as a selectable marker for genetic transformation of plants.Alternative names: PPT N-acetyltransferase, Phosphinothricin-resistance protein.
Purity
Antigen affinity purified serum, in PBS pH 7.4
Reactivity
BAR (BASTA) epitope tag (170-183)
Confirmed reactivity
BAR (BASTA)
Predicted reactivity
Streptomyces viridochromogenes
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 50 µl, of sterile or deionized water.
Expected/apparent MW
20.6 kDa
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 - 1: 5000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Datasheet 