Product Name
DIG | Digoxigenin tag (monoclonal, clone 21H8)
Background
Digoxigenin (DIG) is an hapten from plants (Digitalis) and can be used as an epitope tag in many biological applications.
Purity
Purified on Protein A to a total immunoglobulin fraction in Tris 0.1M, glycine 0.1M with 2 % sucrose.
Reactivity
Digoxigenin (DIG) tag
Confirmed reactivity
Antibody detects free and bound digoxigenin tag
Reconstitution
Reconstitute in distilled water.
Expected/apparent MW
Depends upon fusion partner
Tested applications
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1:1000 - 1:10 000 (ELISA), 1: 200 -1 :2000 (IHC), 1: 500 - 1: 5000 (WB)
Storage
Lyophilized powder is stable for a minimum of 2 years at -20°C. Reconstitute in distilled water before use. Store reconstituted antibodies at 4°C for up to a month and make aliquots for extended storage.
Datasheet 