Product Name
GST-tag (rabbit antibody, polyclonal)
Background
GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Reactivity
GST-tagged proteins
Predicted reactivity
native and denatured forms of purified GST or GST fusion proteins
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
26 kDa
Tested applications
ELISA (ELISA), Western blot (WB)
Recommended dilution
1: 1: 1000 (ELISA), 1: 2000 (WB)
Storage
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 