Product Name
Goat anti-Human IgA+IgG+IgM, HRP conjugated
Immunogen
affinity purified human IgA+IgG+IgM
Background
Goat anti-human IgA+IgG+IgM is a secondary antibody conjugated to HRP which binds to human immunoglobulins in immunological assays.
Purity
Immunogen affinity purified goat IgG.
Reactivity
Human IgA+IgG+IgM
Confirmed reactivity
Human IgA+IgG+IgM heavy chains and light chains on all Human immunoglobulins
Predicted reactivity
Human IgA+IgG+IgM Heavy chains and Light chains on all Human immunoglobulins
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 1.1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Tested applications
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 10 000 -1 : 50 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 50 000 (WB)
Storage
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Datasheet 