Product Name
Donkey anti-Sheep IgG (H&L), HRP conjugated
Immunogen
Purified sheep IgG, whole molecule
Background
Donkey anti-sheep IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all sheep immunoglobulins in immunological assays.
Purity
Immunogen affinity purified donkey IgG.
Reactivity
Sheep IgG (H&L)
Confirmed reactivity
Sheep IgG heavy chains and light chains on all sheep immunoglobulins
Not reactive in
non-immunoglobulin sheep serum proteins based in immunoelectrophoresis
Reconstitution
For reconstitution add 1.1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Tested applications
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 200 -1 : 5 000 (ELISA), 1 : 200-1 : 5 000 (WB) 1 : 500 -1 : 5000 (IHC)
Storage
Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Datasheet 