Product Name
ACT | Actin (recombinant monoclonal, clone 14H4G8)
Immunogen
ca. 100 amino acids of recombinant actin conserved more than 80 % in Arabidopsis thaliana: actin-1 P0CJ46 AT2G37620, actin-2 Q96292 AT3G18780, actin-3 P0CJ47AT3G53750, actin-4 P53494 AT5G59370, actin-5 Q8RYC2 At2g42100, actin-7 P53492 At5g09810, actin-8 Q96293 AT1G49240 , actin-11 P53496 , AT3G12110 ,actin-12 P53497 AT3G46520
Background
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Reactivity
Arabidopsis thaliana, Eschscholzia californica, Medicago sativa, Nicotiana benthamiana, Nicotiana tabacum, Salvia plebeian, Zea mays
Confirmed reactivity
Arabidopsis thaliana, Medicago sativa, Zea mays
Predicted reactivity
Agropyron cristatum, Beta vulgaris, Betula luminifera, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Castanea sativa, Chorispora bungeana, Cyanidioschyzon merolae strain 10D, Glycine max, Glycine soja, Halogeton glomeratus, Medicago truncatula, Malus domestica, Oryza sativa, Pisum sativun, Solanum lysopersicum, Solanum tuberosum, Phaseolus vulgaris, Picea abies, Picea sitchensis, Prunus avium, Ricinus communis, Rubus plicatus, Theobroma cacao,Triticum aestivum, Zea mays, Vicia faba
Not reactive in
Chlamydomonas reinhardtii
Reconstitution
For reconstitution add 50 µl, of sterile or deionized water
Expected/apparent MW
41.6 | 45 kDa
Tested applications
Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1: 500 (IF), 1 : 1000 1: 5000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 