Product Name
AOX1/2 | Plant alternative oxidase 1 and 2
Immunogen
KLH-conjugated synthetic peptide derived from fully conserved C-terminal consensus motif from plant AOX isoforms including Arabidopsis thaliana AOX1A. UniProt: Q39219, TAIR: At3g22370, AOX1B UniProt: O23913, TAIR:AT3G22360, AOX1C UniProt: O22048, TAIR: AT3G27620, and AOX2, UniProt: O22049, TAIR: AT5G64210, Solanum lycopersicum UniProt: Q7XBG9, Oryza sativa UniProt: Q7XT33, AOX1D, TAIR: AT1G32350
Background
Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water.
Reactivity
A. thaliana, B. nana, B. napus, B. oleracea, B. vulgaris, E. vaginatum, H. vulgare, L. luteus, N.tabacum, O. sativa, P.abies, P. sativum, S.tuberosum and T. aestivum, conifers and P. patens cellular[compartment marker] of mitochondrial inner membrane
Confirmed reactivity
Arabidopsis thaliana, Betula nana, Beta vulgaris, Brassica napus, Brassica oleracea, Kandelia candel, Eriphorum vaginatum, Hordeum vulgare, Lupinus luteus, Nicotiana tabacum, Oryza sativa, Picea abies, Pisum sativum, Poa annua, Robinia pseudoacacia, Rosa hybrida, Solanum lycopersicum, Solanum tuberosum, Symplocarpus renifolius, Physcomitrium patens, Tigriopus californicus, Triticum aestivum, Zea mays
Predicted reactivity
Aegilops tauschii, Brachypodium distachyon, Capsella rubella, Citrus sinensis, Citrus clementina, Corylus heterophylla, Crocus sativus, Cucumis sativus, Daucus carota, Glycine max, Hypericum perforatum, Lotus japonicus, Malus x domestica, Medicago truncatula, Medicago sativa, Naegleria gruberi (amoeba), Nelumbo nucifera, Nicotiana benthamiana, Oryza brachyantha, Populus tremula, Picea sitchensis,Pyrus communis,Saccharum officinarum, Sauromatum venosum, Sorghum bicolor, Selaginella moellendorffii, Tetrahymena thermophila, Vigna radiata, Vigna unguiculata, Vitis vinifera
Not reactive in
Candidia albicans, Chlamydomonas reinhardtii (use an antibody to algal AOX1)
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
36-40 | 36-40 for Arabidopsis thaliana
Tested applications
Immunolocalization (IL), Western blot (WB)
Recommended dilution
1 : 750 (IL), 1 : 1000 for 10-20 µg of mitochondrial protein/lane detection (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Datasheet 