Product Name
ADGP | ADP-glucose pyrophosphorylase
Immunogen
part of Arabidopsis thaliana recombinant ADP-glucose pyrophosphorylase small subunit, TAIR: At5g48300, UniProt: P55228
Background
ADP-glucose pyrophosphorylase (AGPase) catalyses the first committed step in the synthesis of transient starch in chloroplasts and storage starch in amyloplasts in plants (Tetlow et al., 2004). AGPase in higher plants is composed of two distinct subunits encoded by separate genes, forming an L2S2 heterotetramer. The large subunits (L) are modulators of allosteric activity, while the small subunits (S) are the catalytic subunits (Kim et al., 2007). Synonymes:ADP-glucose pyrophosphorylase, ADP-glucose synthase, AGPase B, Alpha-D-glucose-1-phosphate adenyl transferase
Reactivity
A. thaliana, C. reinhardtii, H. vulgare, N. tabacum, Polytomella sp., T. aestivum, Z. mays
Confirmed reactivity
Arabidopsis thaliana, Horderum vulgare, Nicotiana tabacum, Triticum aestivum, Zea mays
Predicted reactivity
Chlamydiae, Chlamydomonas reinhardtii, Cyanobacteria, Beta vulgaris, Oryza sativa, Phaseolus vulgaris, Pisum sativum, Planctomycetes, Proteobacteria, Solanum lycopersicum, Spirochetes, Verrucomicrobia, Vitis vinifera
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
49.4 | 52 kDa
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000-1 : 5000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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Datasheet 