Product Name
mCherry (chicken antibody)
Immunogen
Recombinant full length mCherry expressed and purified from E. coli.
Background
mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, however it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).
Purity
Purified, total IgY (chicken egg yolk immunoglobulin) with 3% trehalose.
Confirmed reactivity
mCherry
Predicted reactivity
mScarlett
Reconstitution
For reconstitution add 100 µl of sterile water
Tested applications
Immunocytochemistry (ICC), Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1 : 1000 (ICC), 1: 2000 (IF), 1 : 2000-1 : 5000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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Datasheet 