Product Name
Goat anti-Mouse IgG (H&L), HRP conjugated
Immunogen
Purified mouse IgG (H&L), whole molecule, AAA51107
Background
Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to HRP, which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase mouse IgG (H&L).
Purity
Immunogen affinity purified goat IgG.
Reactivity
Mouse IgG (H&L)
Confirmed reactivity
Heavy chains on mouse IgG and light chains on all mouse immunoglobulins
Not reactive in
Non-immunoglobulin mouse serum proteins
Reconstitution
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily.
Tested applications
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
The optimal working dilution should be determined by the investigator
Storage
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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