Product Name
H1 | Histone H1
Immunogen
native H1 protein purified from Nicotiana tabaccum (H1A, H1B H1C,D,E,F)
Background
Histone 1 (H1) is a protein located in nuclei, incorporated into chromatin.
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Reactivity
A. thaliana, N. tabacum
Confirmed reactivity
Arabidopsis thaliana, Nicotiana tabacum, Triticum aestivum
Predicted reactivity
Lathyrus sativus, Phaseolus vulgaris, Pisum sativum, Solanum lycopersicum, Vicia faba
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
15 | 17 kDa
Tested applications
Chromatin Immunoprecipitation (ChIP), Western blot (WB), Immunocytochemistry (ICC) (ICC)
Recommended dilution
1 : 100-1 : 500 (ICC), 1 : 5000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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Datasheet 