Product Name
PsbA | D1 protein of PSII, phosphorylated
Immunogen
KLH-conjugated synthetic peptide derived from available plant PsbA sequences with phosphorylated (T), including Arabidopsis thaliana UniProt: P83755, TAIR:AtCg00020, Oryza sativa P0C434 and other higher plant PsbA sequences
Background
The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1.
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Reactivity
Global antibody for higher plants
Confirmed reactivity
Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Zea mays
Predicted reactivity
Dictos, Conifers, Monocts
Not reactive in
Cyanobacteria
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
38 | 28-30 kDa
Tested applications
Western blot (WB)
Recommended dilution
1 : 10 000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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Datasheet 