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Corn GMO MON 810 Kit (96)

This is a real-time PCR-based detection kit of MON 810 corn in food, animal feed, and pharmaceutical products.


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Corn GMO MON 810 Kit (96)

Cat#
GMO-105
Product Name
Corn GMO MON 810 Kit (96)
Product Overview
This is a real-time PCR-based detection kit of MON 810 corn in food, animal feed, and pharmaceutical products.
Description
The Corn GMO MON 810 Kit (96) is designed for the target-specific detection of MON 810 corn in food, animal feed, and pharmaceutical products. The assay uses a real-time PCR-based protocol.
Storage
GMO Detection Kit are shipped on dry ice. Multiplex PCR Master Mix and ROX dye should be stored immediately at –20°C upon receipt, in a constant-temperature freezer. All remaining not reconstituted kit components should be stored at 2–8°C and protected from light. Stored under these conditions and handled correctly, assay performance remains unaffected until the date of expiration printed on the quality control label inside the kit box or envelope. Reconstituted reagents of GMO Detection Kit should be dispensed into aliquots to avoid more than 5 freeze–thaw cycles, and stored at 2–8°C for short-term storage (1 month) or –20°C for long-term storage.
Kit Components
PCR Assay MON 810 Corn; Internal Control; Positive Control; Multiplex PCR Master Mix; Nucleic Acid Dilution Buffer; RNase-Free Water; 50x ROX Dye Solution
Scientific Background
The Corn GMO MON 810 Kit (96) was developed for the qualitative detection of the genetically modified organism (GMO) MON 810 corn (one of the most frequently used genetically modified corn lines worldwide) in food and animal feed samples. The corn line MON 810 was developed through a genetic modification resulting in resistance against the European corn borer (Ostrinia nubilalis) by producing Bt‑Toxins of Bacillus thuringiensis. Detection of GMOs using real-time PCR is based on the amplification of a specific region of the relevant GMO genome. The amplified product is detected using target-specific fluorescent probes that bind to it. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the detection of the accumulating PCR product without having to re-open the reaction tubes afterward.
Detection method
Real-time PCR
Features & Benefits
Highly sensitive and specific detection of GMOs containing MON 810 corn; Streamlined detection protocol;
Sample Type
Food, Feed
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