EHA101 Chemically Competent Cell
The genotype of EHA101 Chemically Competent Cell is C58 (rif R) Ti pEHA101 (pTiBo542 D T-DNA) (kan R) Nopaline. Background of EHA101 strain is C58. It contains rifampicin resistant gene (rif) in nuclear genes as screening label. EHA101 carries amber basic Ti plasmid pEHA101 (pTiBo542DT-DNA) to facilitate transformation. Ti plasmid pEHA101 (pTiBo542DT-DNA) contains vir gene, which is essential for insertion of T-DNA into plant genome. Ti plasmid pEHA101 (pTiBo542DT-DNA) is disabled to transfer its own T-DNA but enabled to transfer foreign binary vector T-DNA. pEHA101 (pTiBo542DT-DNA) Ti plasmid contains Kanamycin resistance gene.
EHA101 Chemically Competent Cell is suitable for transgenic operations of corn, rice, tobacco and other plants.
1. Volume of DNA from ligation mix should not exceed 1/10 of the cell mixture; DNA for transformation should be purified and free of organic substances such as ethanol.
2. Do not pipette or vortex cells.
3. Plating volume can be adjusted accordingly.
4. Please avoid excessive use of rifampicin. Maximum concentration of rifampicin in selection is 25 μg/mL.
Store at - 80 °C for 12 months.
EHA101: 100 μL/tube * 10 tube/50 tube/100 tube.
Transformation efficiency of EHA101 Chemically Competent Cell using pK7WGF2 plasmid (spectinomycin resistance) with 50 μg/mL spectinomycin is >10^4 cfu/μg DNA. Transformation efficiency is reduced to half when the plate contains 50 μg/mL spectinomycin and 20 μg/mL rif.