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EHA101 Competent Cell


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EHA101 Competent Cell

Cat#
ACC-108
Product Name
EHA101 Competent Cell
Product Overview
The genotype of EHA101 Competent Cell is C58 (rif r) Ti pEHA101 (pTiBo542 D T-DNA) (kanr) Nopaline. The background of the EHA101 strain is C58 type, and the nuclear gene of EHA101 contains a screening tag - rifampicin resistance gene (rif). In order to facilitate the transformation, the EHA101 strain carries a nopaline-based Ti plasmid pEHA101 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene. The vir gene is an essential component for the insertion of T-DNA into the plant genome, and the T-DNA transfer function of the pEHA101 (pTiBo542DT-DNA) plasmid itself is disrupted, but the foreign binary vector T-DNA can be transferred smoothly. Since the pEHA101 (pTiBo542DT-DNA) plasmid contains the screening tag: kan, the EHA101 strain has kanamycin resistance.
Applications
EHA101 Competent Cell is suitable for transgenic operations of corn, rice, tobacco and other plants.
Storage
Store at - 80 centigrade for 12 months.
Kit Components
EHA101: 100μl/tube * 10 tube/50 tube/100 tube; pK7WGF2(control vector,10ng/μl): 10μl.
Sensitivity
The conversion efficiency of pK7WGF2 plasmid (spectinomycin resistance) in EHA101 Competent Cell is >10^4 cfu/μg DNA.
Sample Type
Corn, rice, tobacco and other plants
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