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GV3101 Electroporation Competent Cell


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GV3101 Electroporation Competent Cell

Cat#
ACC-109
Product Name
GV3101 Electroporation Competent Cell
Product Overview
The genotype of GV3101 Electroporation Competent Cell is C58 (rif r) Ti pMP90 (pTiC58DT-DNA) (gentr) Nopaline. The background of the GV3101 strain is C58 type, and the nuclear gene of GV3101 contains a screening tag - rifampicin resistance gene (rif). In order to facilitate the transformation, the GV3101 strain carries a nopaline-based Ti plasmid pMP90 (pTiC58DT-DNA) without self-transport function, which contains the vir gene. The vir gene is an essential component for the insertion of T-DNA into the plant genome, and the T-DNA transfer function of the pMP90 (pTiC58DT-DNA) plasmid itself is disrupted, but the foreign binary vector T-DNA can be transferred smoothly. Since the pMP90 (pTiC58DT-DNA) plasmid contains the screening tag: gent, the GV3101 strain has gentamicin resistance. GV3101 Electroporation Competent Cell is particularly suitable for large plasmid transformation.
Applications
GV3101 Electroporation Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.
Storage
Store at - 80 centigrade for 12 months.
Kit Components
GV3101 Electroporation: 50μl/tube * 10 tube/50 tube; pCAMBIA2301(control vector,10ng/μl): 10μl.
Sensitivity
The conversion efficiency of pCAMBIA2301 plasmid (size: 11633 bp) in GV3101 Electroporation Competent Cell is >10^5 cfu/μg DNA.
Sample Type
Arabidopsis, tobacco, corn, potatoes and other plants
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