K599 Electroporation Competent Cell

K599 Electroporation Competent Cell

Size Price Qty Online Order
10 tubes (50μL/tube) $648 Order Online  Add to Quote
20 tubes (50μL/tube) $788 Order Online  Add to Quote
50 tubes (50μL/tube) $1,170 Order Online  Add to Quote

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Cat#
ACC-127
Product Name
K599 Electroporation Competent Cell
Product Overview
The genotype of K599 Electroporation Competent Cell is Agrobacterium rhizogenes (str R) pRi2659 (agropine type). Agrobacterium rhizogenes is a Gram-negative soil bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. The K599 Agrobacterium rhizogenes strain contains pRi2659 agrobacterium-type Ri plasmid with a broad host range (cucurbitaceae, leguminosae, solanaceae, etc.) and streptomycin resistance. This competent cell is particularly suitable for the transformation of large plasmids.
Applications
K599 Electroporation Competent Cell is suitable for transgenic operations of cucurbitaceae, leguminosae, solanaceae and other plants.
Notes
1. Volume of DNA from ligation mix should not exceed 1/10 of the cell mixture; DNA for transformation should be purified and free of organic substances such as ethanol.
2. Do not pipette or vortex cells.
3. Plating volume can be adjusted accordingly.
4. Please avoid excessive use of rifampicin. Maximum concentration of rifampicin in selection is 25 μg/mL.
Storage
Store at - 80 °C for 12 months.
Kit Components
K599 Electroporation: 50μL/tube * 10 tube/50 tube.
Transformation efficiency
Transformation efficiency of K599 Electroporation Competent Cell using pCAMBIA2301 plasmid with 50 μg/mL kan is >10^5 cfu/μg DNA. Transformation efficiency is reduced to half when the plate contains 50 μg/mL kan and 20 μg/mL rif.
Our products/services are For Research Use Only. Not For Clinical Use!
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