Product Name
Hygromycin B from Streptomyces hygroscopicus
Product Overview
This product is sourced from treptomyces hygroscopicus. It is plant cell culture tested. CAS NO. 31282-04-9. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.
Description
Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells.
Notes
ygromycin B products should be stored as supplied at 2-8 centigrade, and the dry solid is table for at least 5 years if stored at 2-8 centigrade. It is stable at 37 centigrade for 30 days. (For solutions) This solution is stable as supplied for two years if stored at 2-8 centigrade.
Storage
Store at 2-8 centigrade.
Solubility
50 mg soluble in 1mL of water; ethanol: soluble; methanol: soluble.
Molecular Formula
C20H37N3O13.
Datasheet 