Product Name
Aspergillus PCR Detection Kit
Product Overview
Aspergillus PCR Detection kit is designed to detect Aspergillus flavus, fumigatus, niger, and terreus in cannabis and cannabis-infused products.
Description
The Aspergillus kit is a multiplex test based on gene amplification and detection by real-time PCR. Ready-to-use PCR reagents contain oligonucleotides (primers and probes) specific for A. flavus, A. fumigatus, A. niger, and A. terreus, as well as DNA polymerase and nucleotides.
Storage
This product should be stored at 2-8℃. Reagents stored at this temperature can be used until the expiration date indicated on the tubes.
Warning
This test must be performed by trained personnel.
Samples and enrichment cultures must be handled as potentially infectious material and discarded according to local rules and regulations.
All potentially infectious material should be autoclaved before disposal.
Kit Components
Lysis reagent 20 mL
Fluorescent probes 0.55 mL
Amplification mix 4.4 mL
PCR negative control 0.5 mL
PCR positive control0.25 mL
Lysis beads 17.6 g
Materials Required but Not Supplied
A. Equipment
Lab paddle blender for homogenizing test samples
Incubator for sample microbiological enrichment
Specific for extraction in sterile 1.5 mL conical screwcap tubes
-Benchtop centrifuge capable of 10,000-12,000 × g
-Dry heat block at 37 ± 2℃ and 95-100℃
-Cell disruptor, for example Disruptor Genie
Specific for extraction in deep well plate
-Heating thermoshaker capable of maintaining 37 ± 2℃ and 95-100℃, with a mixing speed of at least 1,300 rpm
-DW 40 Deepwell Microplate Washer
-Benchtop centrifuge with deep well rotor (capable of 2,250 × g)
Vortexer
Magnetic stir plate
20, 200, and 1,000 µL micropipets
Tips for repeat pipettors; sterile, individually packaged
B. Supplies
Enrichment medium
Chloramphenicol
Free DNA Removal Solution
Purification Reagent
Ethanol,96%
Filter sample bags
Powder-free gloves
Distilled sterile wate
Bleach, 5%
Technical Notes
The Aspergillus Kit is a multiplex test based on gene amplification and detection by real-time PCR.
Sample Type
Cannabis and cannabis-infused products
Datasheet 