Product Name
Pollen Viability Test Kit (TTC)
Product Overview
Pollen Viability Test Kit (TTC) is designed for the detection of pollen viability by the TTC staining method.
Description
Pollen viability directly affects the pollination and fertilization process, which is closely related to the plant yield. By measuring pollen activity with our Pollen Viability Test Kit (TTC), you can directly determine the viability of pollen based on color changes, further helping to understand the fertility of pollen.
Detection Method
2, 3, 5-Triphenyte-trazoliumchloride (TTC) color development reaction
Testing Mechanism
2, 3, 5-Triphenyte-trazoliumchloride (TTC) is a redox pigment with a standard oxidation potential of 80 mV. TTC dissolves in water to form a colorless solution, which upon reduction turns into red insoluble 1,3,5-Triphenyltetrazolium formazan (TTF). The respiration of viable pollen is stronger, and the large amount of NADH2 or NADPH2 produced can reduce colorless TTC to red TTF. The respiration of non-viable pollen is weaker and the color of TTC will not change significantly. So we can judge the pollen vigor according to the color change of TTC.
Kit Components
10 mL/50 mL TTC staining solution + an instruction sheet
Storage
Store at 4 ℃, away from light
Applications
Biochemical research
Materials Required but Not Supplied
Slides, coverslips, thermostats and microscopes, etc.
Vigor Judgment Criteria
1. Red color means strong vitality.
2. Light red means weak vigor.
3. Colorless means no vitality or sterility.
Pollen Vigor Calculation Formula
Observe and count 100 pollen grains to calculate the percentage of viable pollen. The calculation formula is as follows：
Percent pollen viability (%) = Number of viable pollen / 100 × 100%
Notes
1. The color should be observed under the microscope immediately after dyeing, and will fade if left for a long time.
2. TTC Stain should be used as soon as possible after opening the lid, otherwise the efficiency will decrease, and should be discarded if it turns red.
3. The pollen needs to be completely submerged in the staining solution when staining.
4. The dyeing temperature is generally 25-35℃.
5. For your safety and health, please wear lab coat and disposable gloves for operation.
Sample Type
Pollen from fresh flowers that are ripe and about to open
Datasheet 