Product Name
Pollen Viability Test Kit (I2-KI)
Product Overview
Pollen Viability Test Kit (I2-KI) is designed for the detection of pollen viability by the I2 -KI staining method.
Description
Pollen viability directly affects the pollination and fertilization process, which further affects the yield of plants. By measuring pollen viability, you can predict the degree of pollen development. The presence or absence and amount of starch contained in pollen can be used as an indicator of pollen activity. Based on this, our Pollen Viability Test Kit (I2-KI) can further help to understand pollen viability by reflecting the amount of starch through color changes.
Detection Method
I2-KI color development reaction
Testing Mechanism
Based on the property that starch turns blue when exposed to iodine, the amount of starch in pollen grains can be determined from the shade of blue, and thus the level of pollen activity. Most normal mature pollen is spherical, containing more starch, and can be stained blue when meeting I2 -KI solution. Poorly developed pollen, on the other hand, is often misshapen and usually contains no or little starch, and does not stain or is stained yellowish-brown when exposed to I2-KI solution. The viability of pollen can be identified according to the pollen staining.
Kit Components
10 mL/50 mL I2-KI staining solution + an instruction sheet
Storage
Store at room temperature, away from light
Applications
Biochemical research
Materials Required but Not Supplied
Slides, coverslips, thermostats and microscopes, etc.
Vigor Judgment Criteria
1. Blue color means strong vitality.
2. Not dyed or dyed yellowish brown color means weak vigor.
3. Colorless means no vitality or sterility.
Pollen Vigor Calculation Formula
Observe and count 100 pollen grains to calculate the percentage of viable pollen. The calculation formula is as follows：
Percent pollen viability (%) = Number of viable pollen / 100 × 100%
Notes
1. After staining, observation under microscope should be done immediately.
2. The pollen needs to be completely submerged in the staining solution when staining.
3. For your safety and health, please wear lab coat and disposable gloves to operate.
Sample Type
Pollen from fresh flowers that are ripe and about to open
Datasheet 