Product Name
Pollen Viability Test Kit (Peroxidase Assay)
Product Overview
Pollen Viability Test Kit (Peroxidase Assay) is designed for the detection of pollen viability by peroxidase staining method.
Description
Pollen viability affects the pollination and fertilization process and is closely related to plant yield. The rapid determination of pollen viability by the color reaction of Pollen Viability Test Kit (Peroxidase Assay) allows experimental prediction and assessment of pollen fertility to aid breeding.
Detection Method
Peroxidase color development reaction
Testing Mechanism
Viable pollen contains an active peroxidase enzyme. Peroxidase can use hydrogen peroxide to oxidize various polyphenols and aromatic amines to produce color, and the activity of pollen can be assessed based on the color change.
Kit Components
1. Reagent (A)
A1: Aniline staining solution (10 mL)
A2: Naphthol staining solution (10 mL)
A3: Aromatic buffer solution (10 mL)
Before use, Reagent (A) needs to be mixed in the ratio A1:A2:A3=1:1:1.
2. Reagent (B) Oxidizer (2*1 mL)
Storage
Store at 4 ℃, away from light
Applications
Biochemical research
Materials Required but Not Supplied
Slides, coverslips, thermostats and microscopes, etc.
Vigor Judgment Criteria
1. Purplish red color means strong vitality.
2. Light red means weak vigor.
3. Colorless means no vitality or sterility.
Pollen Vigor Calculation Formula
Observe and count 100 pollen grains to calculate the percentage of viable pollen. The calculation formula is as follows:
Percent pollen viability (%) = Number of viable pollen / 100 × 100%
Notes
1. The oxidizer is corrosive, so handle carefully to avoid the oxidizer volatilization, otherwise the staining power will be reduced.
2. The pollen needs to be completely submerged in the staining solution when staining.
3. For your safety and health, please wear lab coat and disposable gloves to operate.
Sample Type
Pollen from fresh flowers that are ripe and about to open
Datasheet 