Product Name
β-Glucuronidase Reporter Gene Staining Kit
Category
GUS Staining Kits
General Description
The E. coli GUS gene is extensively used to analyze gene expression in transformed plants. Plants have low intrinsic GUS activity, and the E. coli gene is quite stable in plant cells. As a tag, GUS remains active at the N-terminus of fusion proteins. This kit is used to anaylze β glucuronidase (E. coli GUS gene) expression in transformed plants.
Application
This kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.
Usage
This kit sufficient for 100 histochemical assays.
Features and Benefits
• Histochemical staining of plant tissues expressing the E. coli GUS enzyme.
• Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability.
• GUS does not interfere with plant cell function or viability.
Kit Components
• 2× Fixation Buffer,25 mL.
• 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, ≥98% 1 mL/vial
• Reagent A for gus staining kit,50 mL.
• Reagent B for gus staining kit,200 μL.
• Reagent C for gus staining kit,200 μL.
Storage
Store all reagents at -20°C.
Other Notes
The substrate used in this kit is X-GlcA (5-bromo-4-chloro-3-indolyl β-D-glucuronide) which results in an insoluble indigo-blue precipitate in transfected cells and tissues.
Datasheet 