Product Name
GUS Reporter Gene Kit
Category
GUS Staining Kits
General Description
The β-glucuronidase (GUS) enzyme from E. coli (EC 3.2.1.31) has been well documented to provide desirable characteristics as a marker gene in transformed plants. The GUS reporter gene system has many advantages including stable expression of E. coli GUS enzyme, no interference with normal plant metabolism, and low intrinsic GUS activity in higher plants. The enzyme is also capable of tolerating amino-terminal additions, making it useful for study of plant organelle transport.
Application
This kit allows for quantitative measure of GUS enzyme activity in transformed plants through use of a fluorogenic substrate
Usage
Beta-Glucuronidase (GUS) Reporter Gene Activity Detection Kit
Principle
Enzyme substrates, Enzyme substrates kit for beta-Glucuronidase.
Kit Components
• GUS Extraction Buffer, 25 mL.
• Carbonate Stop Buffer, 25 mL.
• GUS Assay Buffer,5 mL.
• 4-MU calibration stock solution, 5 mL.
Storage
Store kit immediately on receipt. Kit can be stored at 4°C 6 months from receipt, if components have not been diluted. High background fluorescence readings for blank samples will indicate decomposition.
Other Notes
These materials are not included in the kit, but will be required to successfully perform this assay:
• UV-VIS Spectrometer or Plate reader.
• 96-well microplate.
• Bradford Assay (to measure protein concentration).
Datasheet 