Protocols

Transformation of Arabidopsis thaliana

Principle Arabidopsis thaliana is a cruciferous plant, a biennial herb, 7-40 cm tall. It is widely used in research on plant genetics, developmental biology and molecular biology, and has become a typ

Pollen Tube Pathway-Mediated Cotton Transformation

In recent years, with the continuous improvement of transformation technology, the application of pollen tube pathway in agricultural molecular breeding has become more and more widespread. More than

Transformation of Immature Wheat Embryos Using Gene Gun Method

Principle Gene gun method is also called microprojectile bombardment, parti-cle bombardment and biolistics. The gunpowder-detonated gene gun was first developed by Sanford et al. (1987) of Conell Univ

Agrobacterium tumefaciens-mediated Tobacco Leaf Disk Transformation

Principle Transformation of plant cells by Agrobacterium involves a series of complex reactions, including the attachment of Agrobacterium to plant cells, the release of signal molecules from plant ce

Construction of Tomato PIP Subcellular Localization Vector

Expression of some eukaryotic proteins in prokaryotic host cells is efficient and cost-effective. However, many eukaryotic proteins synthesized in bacteria have low or inactive protein activity due to

Construction of Prokaryotic Expression Vector of Cucumber LOX2 Gene

The method of inserting the cloned foreign gene into a suitable vector and then introducing it into E. coli to express a large number of proteins is generally called prokaryotic expression. Prokaryoti

Construction of Soybean NAC Cloning Vector

Cloning vectors are used to clone and amplify target gene fragments in host cells. This type of vector contains a replication signal sequence, which can replicate in host cells to obtain high-copy DNA

Chromosome Walking Technology in Rosa chinensis

Since the Arabidopsis genome has been sequenced, when studying the promoter of a certain gene, primers can be designed based on the known gene sequence and the promoter sequence of the gene can be dir

5'RACE Cloning of The Tobacco Gene SGT1

With the continuous development of biotechnology, many methods and means of cloning new genes have been developed, such as map-based cloning, transposon tagging, differential display of mRNA, genome s

3'RACE Cloning of The Tobacco Gene SGT1

With the continuous development of biotechnology, many methods and means of cloning new genes have been developed, such as map-based cloning, transposon tagging, differential display of mRNA, genome s