Protocols

Polymerase Chain Reaction

Polymerase chain reaction (PCR) is a widely used molecular biology technology used to amplify specific DNA fragments in vitro. It can be regarded as special DNA replication outside organisms. Using PC

Extraction of Soluble Protein from Arabidopsis thaliana Leaves

Soluble proteins refer to proteins that can be dissolved in water or other solvents in the form of small molecules. Most of the soluble proteins in plants are enzymes involved in various metabolisms a

Extraction of Total Protein from Tomato Fruit

Protein is the executor of physiological functions and the direct embodiment of life phenomena. The study of protein structure and function will directly elucidate the change mechanism of life under p

Extraction and Purification of Tobacco Leaf RNA

RNA is an intermediate product of gene expression and also the genetic material of RNA viruses. The manipulation of RNA plays an important role in molecular biology. Highly pure and complete RNA is ne

Extraction of Total Protein from Wheat Leaves

Protein is the material basis of life, the basic organic matter that constitutes cells, and the main carrier of life activities. Without protein there is no life. Therefore, it is a substance closely

Extraction and Purification of Rice Genomic DNA

DNA is the carrier of genetic information and the material basis of gene expression. Whether it is to study the structure and function of DNA or to conduct genetic engineering research, the DNA needs

Extraction and Purification of Plasmid DNA

Plasmid is the most commonly used carrier in molecular cloning, and the preparation of plasmid has become one of the most basic techniques in molecular cloning. The preparation of the plasmid DNA that

Production of Secondary Metabolites By Large-scale Plant Cell Culture

The traditional extraction and processing of plant active ingredients has the following deficiencies: limited by the distribution area of plants; affected by seasons, climate, disease and pest disaste

Selection and Storage of Plant Cell Lines

Screening is the basic method of plant cell selection and improvement. The plant cells obtained by various methods are often inhomogeneous and will show different characteristics (such as early resist

Measurement of Plant Cell Viability

In cell suspension culture, by measuring the viability of cultured cells and cell clusters, we can effectively understand the growth and viability of cells. Before protoplast culture, it is often nece