Protocols

AFLP Analysis of Genetic Diversity and Genetic Relationships of Spinach Germplasm

Spinach belongs to the genus Spinacia of the Amaranthaceae and subfamily Chenopodioideae and is an annual herbaceous plant. Randomly amplified DNA (RAPD) and other technologies have been reported in t

RAPD Analysis of Genetic Diversity for Salt Tolerance in Wheat

Wheat is an annual grass plant. It is restricted due to the scarcity of salt-tolerant germplasm resources. In particular, the salt-tolerance variation of wheat is very limited and the genetic basis is

Detection of Genetic Diversity of Rice Parents by RFLP

Rice is an annual grass plant. Whether it is conventional breeding or hybrid rice breeding, the selection of parents is very important. It requires the parents to carry the target traits or genes and

Separation of Proteins by Two-dimensional Gel Electrophoresis

Two-dimensional electrophoresis is a protein detection technology with higher resolution that combines isoelectric focusing electrophoresis and SDS-PAGE. The gel after two-dimensional electrophoresis

Determining The Relative Molecular Mass of Proteins Using SDS-PAGE

The determination of the relative molecular mass of proteins is of great significance in theory and practice. When a protein is electrophoresed in a polyacrylamide gel, its mobility depends on factors

Analysis of Wheat Peroxidase Isoenzymes by Vertical Polyacrylamide Gel Electrophoresis

In biology, isozymes can be used to study species evolution, genetic variation, cross-breeding, ontogeny, tissue differentiation, etc. Genetic variation in animals and plants can be identified by comp

Separation of Serum Proteins by Cellulose Acetate Membrane Electrophoresis

Cellulose acetate membrane electrophoresis is an electrophoresis method that uses cellulose acetate membrane as a support. It is acetate of cellulose, produced by acetylation of the hydroxyl groups of

Agarose Gel Electrophoresis Detection of Plasmids

Agarose gel electrophoresis is an electrophoresis method that uses agar or agarose as a supporting medium. For samples with relatively large molecular weights, such as macromolecular nucleic acids, vi

Transformation of Tomato

Tomato is a plant of the Solanaceae family and is an annual or perennial herbaceous plant. Tomato is one of the most cultivated vegetable crops in the world. Because it has the advantages of self-poll

Transformation of Cucumber

Cucumber originates from the tropical rainforest areas at the southern foothills of the Himalayas. The botanical classification is Cucurbitaceae. Improving existing cucumber varieties through bioengin