Protocols

Protocols

Tomato Isolated Root Culture Technology

Root culture is mostly used to study root physiological metabolism, organ differentiation and morphogenesis. Root cell cultures can also be subjected to mutagenesis to screen out mutants for plant bre

Media Preparation and Sterilization

Using mother liquor to prepare medium can not only improve work efficiency, but also reduce weighing errors of substances with very small amounts. The medium contains a lot of organic matter, especial

Preparation of Medium Mother Liquor

Before preparing the culture medium according to the culture medium formula, for the convenience of use and accurate dosage, the the macroelements, trace elements, iron salts, organic matter, and horm

Artificial Seed Preparation

Artificial seed is a capsule structure which consist of an embryoid produced by tissue culture techniques and protective substances that can provide enough nutriments and keep the embryoid from spoili

Suspension Culture

Suspension culture is a tissue culture system for culturing single cells and small cell clusters in a constantly agitated or shaken liquid medium, which is used to culture non-adherent dependent cells

Chemical Treatment for Detoxification

Viral diseases have been an important constraint to the sustainability of plant production. The use of virus-free seedlings is one of the effective ways to prevent and control plant virus diseases. De

Ultra-low Temperature Detoxification

Ultra-low temperature detoxification is a new and efficient detoxification technology based on ultra-low temperature preservation. Compared with traditional detoxification methods, ultra-low temperatu

Heat Treatment Combined with Stem Tip Detoxification

Plants, especially economic species, are plagued by viruses that lead to low yields and poor quality, and plant stem tip detoxification is one of the common countermeasures to address this problem. Pl

Micro Stem Tip Detoxification

Plants grown in their natural environment carry a wide range of viruses and this has caused huge economic losses. It is generally believed that in stem tip micro stems, the vascular system is not well

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