Food Safety and Nutrient Detection

Total Collagen Assay Kit II (Perchlorate-Free) is a quick and convenient protocol for detection of small amounts of collagen in a variety of biological samples. The assay is based upon hydrolysis of samples to yield free Hyp, which is oxidized and reacted to give a brightly-colored chromophore with an absorbance peak at 560 nm. The assay employs a proprietary acidic developer solution to accurately measure collagen in hydrolysates without the use of hazardous perchloric acid, obviating the need for any special handling and waste-disposal protocols. The assay can detect a minimum of 0.5 µg collagen per well in a 96-well format and generates colorimetric results that are virtually identical to those of the classical perchloric acid-based method.

Tyrosine Assay kit is a simple, yet sensitive assay that is able to detect normal and abnormal concentrations of Tyr in biological fluids. The assay is based on the enzymatic oxidation of Tyr producing a stable signal (OD 492 nm), which is directly proportional to the amount of Tyr. Sample preparation is minimal and does not require strenuous or complicated procedures. The assay can detect as low as 50 µM of Tyr in a variety of biological samples.

The kit is an easy and accurate assay to measure β-HB levels in biological samples. The assay is linear for 1-20 nmol β-HB in up to 100 µl samples or 0.01-0.2 mM of β-HB samples.

The kit is an easy and accurate assay to measure β-HB levels in biological samples. The assay is linear for 1-20 nmol β-HB in up to 100 µl samples or 0.01-0.2 mM of β-HB samples.