DNA carries genetic information. The most used DNA extraction methods are phenol/chloroform/isopentyl alcohol, CTAB, and SDS methods, which require multiple extractions of organic reagents to remove proteins and polysaccharides, thus obtaining high-quality DNA. Mollusk shells are superior as a DNA source for genetic analysis compared to mollusk tissues. Lifeasible can perform shell DNA extraction experiments and evaluate the quality of the obtained genomic DNA by micro-UV spectrophotometry and agarose gel electrophoresis analysis.
Lifeasible offers high quality molluscan DNA extraction services with an experienced laboratory team, well-equipped facilities, and a high level of automation. Choose us if you have a large, time-consuming, and labor-intensive project to complete. We can provide cost-effective solutions to meet your project needs, and we can also provide a range of scientific services based on sequencing and more.
The mollusks were submerged in 15% EDTA decalcification solution for about 2 h at 4 °C to remove impurities from the shell surface, then washed with 4×PBS buffer and placed in an incubator to dry the water. Weigh a quantitative number of mollusks into liquid nitrogen, quickly and simply chopped, and then ground on a medium-throughput tissue grinder for 1-2 min to obtain powder samples, added to lysis solution, and then put into a molecular hybridization oven for lysis; at room temperature, centrifuged, aspirated supernatant added to EP tube, then added -20 °C pre-cooled anhydrous ethanol and sodium acetate, slowly and gently shaken to precipitate. Centrifuge and obtain DNA precipitate. Discard the supernatant, add pre-chilled ethanol, gently pop up the precipitate at the bottom of the tube by hand, centrifuge, discard the supernatant, and repeat once.
The DNA precipitate was dried in a fume hood, and deionized water was added to dissolve the DNA. 10-fold dilutions of the stock solution were used to determine the concentration of DNA and for agarose gel electrophoresis.
Using an ultra-micro-UV spectrophotometer, the concentration of molluscan shell DNA samples and the absorbance at 260 and 280 nm were measured, respectively, and the DNA purity was determined based on the ratio of OD 260/280. The appropriate amount of DNA extract and buffer were mixed, spotted on an agarose gel with electrophoresis buffer at constant pressure and electrophoresis for 25-30 min. The results were observed and photographed with a gel imaging analysis system.
In addition to the above-mentioned molluscan DNA extraction services and genomic DNA quality testing services, we can also provide professional solutions, specific experimental steps, experimental protocols, instrument parameters, and other content.
If you are interested in our services, or if the service you want is not listed, please feel free to contact us, and we will get back to you as soon as possible.
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