Food Safety and Nutrient Detection

BCAA Assay Kit provides a simple convenient means of measuring the BCAA’s in a variety of biological samples. The kit utilizes an enzyme assay in which BCAA is oxidatively deaminated, producing NADH which reduces the probe, generating a colored product (λmax = 450 nm). BCAA kit measures BCAA’s in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 µM BCAA in sample). BCAA’s are present in serum ~ 0.1-0.4 mM each (~0.125-1.5 mM combined).

Cysteine Assay Kit provides a simple, sensitive, and high-throughput adaptable assay that detects physiological concentration of cysteine in a variety of biological fluids. The principle of the assay is based on the cleavage of thiol group of reduced cysteine producing a fluorophore (Ex/Em = 365/450 nm) with a stable signal, which is directly proportional to the amount of total cysteine in the sample. The assay is specific and other thiol-based amino acids do not interfere with the assay. The assay can detect as little as 10 µM of Cysteine in a variety of samples.

L-Serine is one of the 20 naturally-occurring amino acids used by all organisms in the biosynthesis of proteins. L-Serine (L-Ser) is a non-essential amino acid In addition to its role as a protein building block, L-Serine serves as a precursor to many vital biomolecules, including phosphatidylserine, sphingomyelin,and the amino acids glycine and cysteine.L-Ser is importantfor brain development and functions which areespecially sustained by altered L-Ser levels inpsychiatric disorders and occurrence of severeneurological abnormalities in patients withdefects in L-Ser biosynthesis.

Fructosamine Assay Kit is a microplate-based colorimetric assay for the direct determination of fructosamine levels in serum. The assay is based on the ability of fructosamine to reduce nitroblue tetrazolium (NBT), forming a colored end-product (purple) under alkaline conditions. The formation rate of formazan is proportional to the concentration of fructosamine in samples and the increase in absorbance (OD 530nm) can be monitored using a spectrophotometer. The kit includes a Thiol Blocking Reagent and a Sample Cleaning Mix that minimizes the interference of other endogenous reducing agents which ensures accurate measurements of Fructosamine in biological samples. The assay is simple, reproducible and can detect as low as 20 µmol/L of fructosamine in samples.

Fumarate Assay Kit provides a convenient tool for sensitive detection of the fumarate in a variety of samples. The fumarate Enzyme Mix recognizes fumarate as a specific substrate leading to proportional color development. The amount of fumarate can therefore be easily quantified using a colorimetric assay (λ = 450 nm). It can detect as low as 1 nmol of fumarate per well (20 µM).

In the assay kit, Galactose is specifically oxidized generating a product that produces color (O.D 570 nm) and fluorescence (Ex/Em 535/587 nm). Liquid samples can be tested directly without purification. The assay is fast, convenient and sensitive. It can also be used as a high throughput assay.

The Glucose-6-phosphate Assay Kit can detect G6P in the range of 1 to 30 nmoles with detection sensitivity ~10 µM of G6P.

Glutamine Colorimetric Assay kit is a simple and sensitive assay that detects the biologically relevant concentrations of Gln in various fluids and tissues. The assay is based on the hydrolysis of Glutamine to Glutamate producing a stable signal, which is directly proportional to the amount of Gln in the sample. The assay can detect as little as 25 µM of Gln in a variety of biological samples.

Glycerophosphorylcholine Assay Kit allows a quick and sensitive means to determine GPC concentration in a biological sample. The assay is robust and specific, with a GPC enzyme mix converting GPC in the sample into an intermediate that is then utilized to quantitatively generate signal using the GPC Developer mix and GPC Probe. The included Sample Clean-Up Mix allows the user to avoid high background from choline, which is present at high levels in many biological fluids. The assay is rapid, simple, high throughput compatible, and can detect GPC concentrations as low as 0.1 µM in tissue lysates and other samples such as breast milk and seminal plasma.

Glycine Assay Kit provides a simple, sensitive, and high-throughput adaptable assay that detects physiological concentration of glycine in a variety of biological fluids. The principle of the assay is based on the oxidation of glycine producing a fluorophore (Ex/Em = 535/587 nm) with a stable signal, which is directly proportional to the amount of GLY in the sample. The assay is specific and other standard and non-standard amino acids do not interfere with the assay. The assay can detect as little as 1 µM of Glycine in a variety of samples.