Food Safety and Nutrient Detection

In this assay, glucose is specifically oxidized to generate a product which reacts with a dye to generate color (λ = 450 nm) whose intensity is proportional to glucose concentration. The method is rapid, simple, sensitive, and suitable for high throughput. This assay is particularly suitable for serum and urine samples since it is unaffected by reducing substances which can interfere with other suppliers offering oxidase-based kits. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes. The kit can detect glucose concentrations in the range of 20µM - 10mM.

The Glucose Assay Kit provides direct measurement of glucose in various biological samples (e.g., serum, plasma, body fluid, food, growth medium, etc.). Glucose Enzyme Mix specifically oxidizes glucose to generate a product which reacts with a dye to generate color (λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The generated color and fluorescence is proportionally to the glucose amount. The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes. The kit detects 1-10000 µM glucose samples.

Glutathione Detection Kit provides a unique, convenient tool for detecting GSH, GSSG, and total glutathione individually. In the assay, OPA, reacts with GSH (not GSSG), generating fluorescence, so GSH can be specifically quantified. Adding a reducing agent converts GSSG to GSH, so (GSH + GSSG) can be determined. To measure GSSG specifically, a GSH Quencher is added to remove GSH, preventing reaction with OPA (while GSSG is unaffected). Reducing agent is then added to destroy excess quencher and to convert GSSG to GSH. Thus, GSSG can be specifically quantified. The kit provides a unique procedure and buffer formula to eliminate protein thiol interference and to stabilize GSH and GSSG in solution.

Glutathione Detection Kit provides a simple in vitro assay for detection of total glutathione changes in apoptosis and other samples. The assay utilizes monochlorobimane (MCB), a dye that appears to form an adduct exclusively with glutathione. The unbound MCB is almost nonfluorescent, whereas the dye fluoresces blue (Ex./Em. = 380nm/461nm) when bound to glutathione of reduced or oxidized form. The reaction is catalyzed by glutathione S-transferase. Thus, the amount of total glutathione can be easily detected using a fluorometer or a 96-well fluorometric plate reader.

Glycerol Cell-Based Assay Kit provides a simple, sensitive, straight-forward assay for determination of glycerol concentrations in various cell and tissue culture samples. The assay uses a glycerol-specific enzymatic reaction to convert the probe into a fluorescent product in the presence of glycerol. Glycerol levels can be detected as low as 50 pmol per well, or 1.0 µM in culture medium. Chloroquine (included in the kit as a control) induces steatosis and blocks autophagy, thus leading to a buildup of lipid droplets and an increase in the free glycerol concentration.

In the assay, cholesterol oxidase specifically recognizes free cholesterol and produces products which react with probe to generate color (570 nm) and fluorescence (Ex/Em = 538/587 nm). Cholesterol esterase hydrolizes cholesteryl ester into free cholesterol, therefore, cholesterol ester and free cholesterol can be detected separately in the presence and absence of cholesterol esterase in the reactions.

Heme is a complex molecule with an iron atom at its center, surrounded by a porphyrin ring. This structure enables heme to bind with oxygen and other molecules, making it essential for oxygen transport and carbon dioxide removal in the blood. In medical examinations, heme is crucial for several tests. Measuring hemoglobin levels assesses the blood's oxygen-carrying capacity. The presence of heme in urine can indicate conditions such as hemolytic anemia, renal diseases, and certain cancers.

Iron Assay Kit provides a simple convenient means of measuring Ferrous and/or Ferric ion in sample. In the assay, ferric carrier protein will dissociate ferric into solution in the presence of acid buffer. After reduction to the ferrous form (Fe²ᶧ), iron reacts with Ferene S to produce a stable colored complex and give absorbance at 593 nm. A specific chelate chemical is included in the buffer to block copper ion (Cu²ᶧ) interference. The kit measures iron in the linear range of 0.4 to 20 nmol in 50 µl sample, or 8 µM to 400 µM iron concentration in various samples.

In the Lactate Assay Kit, lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a probe to produce a color (λmax = 450 nm). The kit detect L(+)-Lactate in biological samples such as in serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples. It detects 0.02 mM – 10 mM lactate in various samples.

The kit provides a convenient means for detecting L(+)-Lactate in biological samples such as in blood circulation, in cells, in culture mediums, in fermentation mediums, etc. There is no need of pretreatment or purification of samples. The kit can detect 0.001-10 mM of various Lactate samples.