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Food Safety and Nutrient Detection


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Food Safety and Nutrient Detection Inquiry
Cat#:
AgK-366
Product Type:
Food Safety and Nutrient Detection
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Cat#:
AgK-367
Product Type:
Food Safety and Nutrient Detection
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Cat#:
AgK-369
Product Type:
Food Safety and Nutrient Detection
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Methanol Assay Kit utilizes an enzymatic mechanism by which conversion of methanol is correlated stoichiometrically with generation of a colorimetric signal that can be quantified at 450 nm. The assay shows greater than 100-fold specificity for methanol over ethanol. The method is suitable for use in a range of biological and consumable samples, and can detect as little as 500 pmol methanol.
Cat#:
AgK-370
Product Type:
Food Safety and Nutrient Detection
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Nickel Assay kit provides a simple method of quantifying Ni²ᶧ in a variety of samples. The assay takes advantage of reaction of Ni²ᶧ with mercaptoethanol in borate buffer to form a complex with strong absorbance bands from ~300 to 600 nm. Fe²ᶧ and Co²ᶧ interfere with the assay, therefore extra steps (as described below) must be taken to subtract the interference in order to determine the correct Nickel concentration in mixed samples. Other ions tested (Mn²ᶧ, Cu²ᶧ, Zn²ᶧ) do not interfere with the assay, presumably no other ionic species are present in high enough concentration to interfere with the reaction. The assay is a simple method of quantifying Ni²ᶧ in a variety of samples, which gives a linear range of 2 to 50 nmol Nickel containing less than 25 nmol Cobalt.
Cat#:
AgK-371
Product Type:
Food Safety and Nutrient Detection
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Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process. The first step converts nitrate to nitrite utilizing nitrate reductase. The second step uses Griess Reagents to convert nitrite to a deep purple azo compound. The amount of the azochromophore accurately reflects nitric oxide amount in samples. The detection limit of the assay is approximately 1.0 nmole nitrite/well, or 10 µM.
Cat#:
AgK-376
Product Type:
Food Safety and Nutrient Detection
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Phosphate Colorimetric Assay Kit provides an easy, quick and sensitive means of assessing phosphate over a wide range of concentrations. The assay utilizes a proprietary formulation of malachite green and ammonium molybdate which forms a chromogenic complex with phosphate ion giving an intense absorption band around 650 nm. Phosphate concentrations between 1 µM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined. The Phosphate Colorimetric Assay Kit provides 500 assays using microtiter plates or 100 assays using 1 ml cuvettes.
Cat#:
AgK-377
Product Type:
Food Safety and Nutrient Detection
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This kit offers an excellent and efficient method for high-throughput assay that can detect phospholipid in the range of 0.1 to 10 nmol per sample
Cat#:
AgK-378
Product Type:
Food Safety and Nutrient Detection
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Cat#:
AgK-379
Product Type:
Food Safety and Nutrient Detection
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Pyrophosphate (PPi) Assay Kit provides a fast, convenient and ultrasensitive method for determination of free inorganic pyrophosphate levels in biological material. PPi produced during biotic processes is detected through a series of reactions which utilize a proprietary enzyme mix and probe, generating a stable product that can be quantified by either colorimetric or fluorometric readout. Generated fluorescence (Ex/Em = 535/587 nm) or color (OD: 570 nm) intensities are directly proportional to the concentrations of pyrophosphate, enabling precise measurements. Monomeric inorganic phosphate (Pi) does not interfere with the assay. Our assay delivers an easy and robust method suitable for use in a variety of biological samples and can be performed in a convenient microtiter-plate format. The kit provides sufficient reagents for 100 fluorometric or 50 colorimetric assays, respectively. This kit can detect as low as 1.8 μM PPi in plasma and serum samples.
Cat#:
AgK-382
Product Type:
Food Safety and Nutrient Detection
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ROS Detection Assay Kit is designed for detection of hydroxyl, peroxyl, or other reactive oxygen species in live cells. We utilize H2DCFDA, a unique cell-permeable fluorogenic probe, compatible with phenol red, FBS and BSA to detect reactive oxygen species in live cells. Upon the cell entry, H2DCFDA is modified by cellular esterases to form a non-fluorescent H2DCF. Oxidation of H2DCF by intracellular ROS yields highly a fluorescent product that can be detected by FACS, microplate reader, or fluorescence microscope (Ex/Em 495/529 nm). The fluorescence intensity is proportional to the ROS levels. Our kit provides a simple and specific assay for the real-time measurement of global levels of ROS in living cells. We include sufficient reagents to perform 100 assays and a common ROS inducer as a control for measurement of ROS levels or antioxidant activity with high sensitivity, specificity and accuracy
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