Analysis of Reactive Oxygen Species in Plants

The rapid production of reactive oxygen species (ROS), such as superoxide anion and hydrogen peroxide, is a conserved signaling response across kingdoms, and in plants, it is induced at an early stage of plant nematode infection. ROS have direct antimicrobial properties but also serve as signaling molecules to activate additional and complementary immune outputs such as strengthening cell walls by cross-linking polymers, amplifying and propagating intra- and intercellular defense signals, and regulating hypersensitive response (HR)-cell death.

Lifeasible is a credible provider of comprehensive, high-quality services for plant science. We provide an advanced and professional platform for analyzing ROS in plants according to the customers' needs. We hope to partner with you to explore new frontiers in plant nematodes and accelerate your discovery.

Positive Correlation of ROS with Nematode Resistance

• At the biochemical level, the rapid generation of reactive oxygen species (ROS), such as superoxide (O^2-) and hydrogen peroxide (H₂O₂), the oxidative burst, is the first plant reaction in response to the attack by an avirulent or virulent pathogen. Many studies have reported that the occurrence of ROS has been shown to correlate positively with enhanced resistance to nematodes.
• In incompatible interactions between plant nematode infection and resistant plants, this nonspecific, weak, and transient ROS production is followed by a massive and prolonged ROS accumulation, and the latter is intimately associated with the HR response.
• Lifeasible provides analysis services for the positive correlation of ROS with nematode resistance, including detection of peroxidase and superoxide dismutase activities in plants using enzymatic assays, subcellular localization of ROS, the intensity, and duration of the oxidative burst, etc.

Fig.1 ROS-induced oxidative stress in nematodes.

Involvement of ROS in Basal Defense

• It is reported that the direct involvement of ROS in basal defense. The plasma membrane-bound NADPH oxidase respiratory burst oxidase homologs (RBOHs) are important for producing apo plastic ROS.
• We provide analysis services for the involvement of ROS in basal defense, including characterizing the role of Rboh-mediated ROS during compatible interaction between plants and nematodes, the susceptibility of mutants in response to plant nematodes, and the stimulative effect on the expression of antioxidant genes in the infected tissue as a signaling molecule. Besides we also provide an analysis of RBOHs and their homolog, such as whitefly-induced 1 (WFI1) in tomatoes.

With years of experience in biological services, our advanced platforms can help our clients solve various difficulties in the research of plant nematodes. Lifeasible provides our clients with direct access to our experts and responses to their questions. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.