Brazzein is a small, sweet protein originally isolated from the West African climbing vine Pentadiplandra brazzeana Baillon. Brazzein has a high level of sweetness potency and is stable in extremes of temperature and pH. Brazzein has received a lot of attention as a potential replacement for traditional sweeteners in the food and beverage industry. Developing efficient strains for the large-scale production of brazzein is crucial for its use in food and medicine.
Lifeasible is a leading biotechnology company with extensive experience in developing production strains to produce various proteins and enzymes. Brazzein production strain development service is one of our specialties, and we provide customized services according to customers’ specific needs.
Lifeasible offers a comprehensive service for the development of brazzein-producing strains. Our team of experienced scientists can assist you in selecting the best combination of production strains and plasmids to ensure the high-level expression of Brazzein. We also offer a comprehensive optimization service to determine the best expression conditions for your specific strain and plasmid combination.
| Service Step | Service Content |
| Microbial strain selection | According to your research purpose, select the appropriate microbial strain, or develop multiple strain production systems simultaneously. |
| Construction of the brazzein expression vectors | Vectors such as pPpT4_Alpha_S can be used to express brazzein. The gene sequence of brazzein is amplified, a suitable restriction site is selected, and the brazzein gene is cloned into the vector. If necessary, we will also optimize codons according to the target strain and plasmid. |
| Transformation and optimization of culture conditions | Transfer brazzein into the target strain by plasmid-mediated or electrotransformation. Then we optimize the culture conditions of the strain through a series of measures, such as carbon source, nitrogen source, and other nutrients, etc., to finally improve the expression of brazzein. |
| Analysis of recombinant brazzein | Purify the recombinant brazzein expressed by the microbial strain and then analyze and verify it by WB and mass spectrometry. We are also able to analyze the function of recombinant brazzein using human embryonic kidney cells (HEK293T) stably transfected with chimeric Gα16gust44 protein. |
Technical means: plasmid transformation and electroporation method
Microbial strains can be developed: Escherichia coli, Lactococcus lactis, Bacillus subtilis, Saccharomyces cerevisiae, Pichia pastoris, Kluyveromyces lactis, etc.
Vectors: plasmid pMSP3545, plasmid pNZ8148, pPpT4_Alpha_S vector, etc.
Delivery results: microbial strains capable of expressing brazzein, experimental data, pictures, and standard experimental reports.
As a top biotech company, Lifeasible is proud to offer our high-quality Brazzein production strain development services. Our team of experienced scientists is dedicated to ensuring your complete satisfaction. For more information, please contact us directly.
Reference
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