About 500 times sweeter than sucrose, neoculin is a low-calorie, high-intensity sweetener. The practical application of neoculin will help patients suffering from diseases such as obesity and diabetes. Neoculin has both sweetness and taste-modifying activity, qualities that make it an attractive alternative to traditional sugar substitutes. However, the limited sources of neoculin limit its application in the food industry. The production of neoculin using microbial strains is gaining popularity.
Lifeasible is committed to providing high-quality and customized neoculin production strain development services to meet customers’ needs. Our team of experienced experts is dedicated to ensuring the success of your project from strain selection to development.
With over 20 years of experience, we offer genetically modified bacteria or yeasts to express neoculin proteins. Screening microbial strains, optimizing neoculin gene expression, optimizing strain culture conditions, etc. With a comprehensive suite of services, we can help our customers obtain strains of interest faster and more efficiently than ever.
| Service Step | Service Content |
| Microbial strain selection | In addition to customer-determined strains, we can also choose microbial strains according to your purpose. Bacteria and fungi are all optional content for us. |
| Construction and amplification of neoculin expression plasmid | Select the appropriate restriction site according to the gene sequence, clone the neoculin gene, and connect the gene to the expression plasmid. The constructed plasmid is transferred to E.coli DH5α for amplification. |
| Transformation of strains | Using the transformation test, the plasmid expressing neoculin is transferred into the target strain. Transformants are then screened for neoculin production. We will also optimize temperature, medium, trace elements, etc., and explore the culture conditions conducive to large-scale production of neoculin. |
| Purification of recombinant neoculin | We precipitate neoculin in a liquid medium, then purify and collect neoculin by dialysis, filtration, and chromatography for further analysis. |
| Neoculin characterization | We will prepare anti-neoculin antibody to detect both recombinant neoculin and natural neoculin. |
| Tests of sweetness response and sensory analysis | We culture human embryonic kidney cells expressing human sweet taste receptors and performed Ca2+ imaging on them to analyze the sweetness of recombinant neoculin; sensory tests will also be used to verify the flavoring activity of recombinant neoculin. |
Technical means: plasmid transformation
Microbial strains can be developed:Escherichia coli, Aspergillus oryzae, Saccharomyces cerevisiae, etc.
Vectors: pEAK10 expression vector, etc.
Delivery results: microbial strains capable of expressing neoculin, experimental data, pictures, and standard experimental reports.
Lifeasible is committed to providing high-quality and custom neoculin production strain development services to meet our client's needs. Our team of experienced experts is dedicated to ensuring the success of your project. For more details, please feel free to contact us.
Reference
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