Detection of Plant Nematodes Based on Molecular Biology

In the case of overlapping morphological characteristics of nematodes, identification with examiners' subjectivity is often inaccurate. Thus, molecular approaches have advanced over classical or traditional taxonomic approaches in identifying nematodes. Polymerase chain reaction (PCR) is widely established and effectively used to classify nematodes. In past studies, high-quality research results have been obtained using these molecular markers.

Lifeasible provides the detection of plant nematodes based on molecular biology, including PCR, molecular markers, loop-mediated isothermal amplification, and recombinase polymerase amplification. Our experts will answer your questions comprehensively and carefully and help you solve the problems in your studies. We guarantee satisfied and reliable results for our customers all over the world.

Detection of Plant Nematodes by PCR

• Currently, PCR-based detections are important and reliable sources for classifying and characterizing new species in nematode taxa.
• Compared to the traditional diagnostic process, PCR-based detection technologies have revolutionized the field of nematode diagnosis, with advantages in sensitivity, specificity, speed, relative ease of use, and cost-effectiveness.
• Lifeasible provides conventional PCR, nested PCR, and real-time PCR to assist our customers in the detection of plant nematode.

Fig.1 Different molecular markers for the identification of plant nematodes.

Detection of Plant Nematodes by Molecular Markers

• Many kinds of molecular markers are developed based on DNA / PCR technology, which can be used for nematode detection. They are effective tools for analyzing the genetic variation of plant nematode populations both among species and under species.
• We provide detection of plant nematodes based on molecular markers for our customers, mainly including restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), sequence characterized amplified region (SCAR), etc.

Detection of Plant Nematodes by Loop-Mediated Isothermal Amplification

• Loop-mediated isothermal amplification (LAMP) is a new nucleic acid amplification method which requires the design of 4 - 6 primers, including external primers and internal primers. Under the constant temperature of 60 - 65°C, many target fragments are amplified rapidly.
• We provide detection of plant nematodes based on LAMP, which not only has the characteristics of simple operation, low cost, and easy equipment requirements but also has a wide range of applications and good results in the detection of nematodes and other aspects.

Detection of Plant Nematodes by Recombinase Polymerase Amplification

• Recombinase polymerase amplification (RPA) technology mainly relies on recombinant enzymes, single-strand binding proteins, and strand displacement DNA polymerases to achieve exponential amplification of the target region on the template.
• We provide nematode detection based on PRA technology, which can realize field detection and has relatively accurate results.

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