Thaumatin Transgenic Plants Development

Thaumatin was isolated from the fruit of the West African tropical plant Thaumatococcus daniellii Benth in 1972. Thaumatin contains 207 amino acids and has a molecular weight of 22,200 Da. There are two major forms (I and II) and three minor forms (a, b, c). Only higher primates can taste thaumatin, a protein that tastes very sweet. Thaumatin is approximately 10,000 times sweeter than sugar on a molar basis. At present, thaumatin has been approved for use in food, medicine, and cosmetics in China, the United States, the United Kingdom, Japan, Germany, and other countries and regions. Due to the superiority of thaumatin in replacing traditional sugars, more and more scientists are trying to transfer it to plants.

Having worked on genetic modification for many years, Lifeasible deeply understands sweet proteins. Our scientists have developed an excellent system and advanced technology platform, which can provide customers with a one-stop thaumatin transgenic plant development service.

What We Offer?

Our R&D service can help customers obtain thaumatin transgenic plants that induce a sweet phenotype and plant bioreactors that produce thaumatin. We typically use E. coli to clone and amplify the thaumatin gene. We use a binary vector to transfer the gene into an Agrobacterium strain and transform it into the target plant.

Service Content

Technical means: Agrobacterium-mediated transformation

Agrobacterium tumefaciens strains: A4T，CBE21，LBA4404，C58C1, etc.

Vectors: shuttle vector pWIT2，binary vector pGA482GG/35S-THA2-ocs3，binary vector pCAMBIA1301，pGEM B1, etc.

Plant types that can be transformed: tobacco (Nicotiana plumbagnifolia), rice (Oryza sativa), strawberry (Fragaria × ananassa), apple (Malus domestica), cucumber (Cucumis sativus), potato (Solanum tuberosum), pear (Pyrus communis), tomato (Solanum lycopersicum), carrot (Daucus carota subsp. sativus), celery (Apium graveolens), hyacinth (Hyacinthus orientalis), etc.

Delivery results: at least 10 T0 positive thaumatin transgenic plants, experimental data, pictures, standard genetic transformation experiment report.

Flowchart for Ordering Service

Our Advantages

• Thaumatin can be post-translationally modified to enhance biological activity and stability.
• With rich and successful research and development experience, plants can be transformed, including crops, fruits, vegetables, etc.
• It can be grown large using traditional agricultural methods, reducing production costs and environmental impact.

Lifeasible is committed to overcoming all transgenic difficulties and providing you with a complete range of thaumatin plant solutions. For more information or cooperation intention, please feel free to contact us.