Monellin Production Strains Development

Monellin has a series of unique advantages such as safety, low calorie, no unnatural metabolites introduced into the body, maintenance of amino acid pool balance, and relatively simple cloning into microorganisms. It has the potential to be used as a food additive or drug. The sweetness of monellin is pH dependent, and the protein is odorless at pH < 2 and pH > 9. In the United States, monellin is classified as a food additive not generally recognized as safe (GRAS); however, in the European Union and Japan, monellin is considered a novel food additive that can be used in food after safety assessment, and regulatory approval.

As one of the most promising sweet proteins, monellin has received extensive attention. However, extracting monellin from natural sources is costly, and the plants are difficult to cultivate. Therefore, scientists sought ways to express monellin in microorganisms. The increase in obesity and diabetes has also accelerated the demand for the industrial production of monellin. As a plant biological service CRO, Lifeasible provides monellin production strains development service to customers.

What We Offer?

Expression of monellin in microbial systems is a feasible and efficient approach that can provide large and stable amounts of monellin whose sweetness and functional properties can be tuned as desired. At present, we have successfully developed monellin-expressing microbial systems for many customers. Different microbial expression systems have various advantages and disadvantages; we need to select and optimize according to the customer's target strains and application scenarios.

Service Content

Technical means: plasmid transformation

Microbial strains that can be developed: Saccharomyces cerevisiae,food yeast Candida utilis, Pichia pastoris, Escherichia coli, Bacillus subtilis, etc.

Vectors: pCU1 plasmid, pETDUET plasmid, pHPMS plasmid, etc.

Delivery results: microbial strains capable of expressing monellin, experimental data, pictures, and standard experimental reports.

Flowchart for Ordering Service

Our Advantages

• The strains developed allow large-scale production and purification of monellin, reducing costs and increasing efficiency.
• We can enhance the stability and sweetness intensity of monellin by changing its amino acid sequence or linkage.
• Our scientists can construct food-grade expression systems for you, avoiding the use of elements that may cause safety problems, such as antibiotic resistance genes.

Lifeasible, as a professional biotechnology company, is oriented to the needs of our customers and develops solutions that meet all their needs. We will do our best to provide our customers with scientific, accurate, and realistic experimental results. If you need to develop a microbial strain for monellin production, please feel free to contact us.