Mollusk Mitochondrial Sequencing Services

The mitochondrial genome structure of mollusks shows a high degree of variability involving many genomic features, i.e., length, gene arrangement, strand assignment, gene duplication and loss, nucleotide composition, etc. It shows extensive variation in mollusks, gastropods, and bivalves, even within the same family or genus. Mitochondrial DNA (mtDNA) has been widely used in studying biological evolution and population structure due to its unique maternal inheritance pattern and high mutation frequency. Second-generation sequencing can obtain mitochondrial DNA sequence information easily and rapidly with high throughput; therefore, molluscan mitochondrial sequencing is critical in the field of evolutionary biology research.

Background

• First-generation Sanger sequencing

Mitochondria DNA sequencing is usually performed on the products of mitochondrial signature gene amplification, as well as on the smaller fragments of Gap present in the second-generation random sequencing for loop formation.

• Second-generation Illumina random sequencing

Usually, for most animal-like mitochondria and relatively small mitochondrial genomes.

• Third-generation PacBio long fragment sequencing

a) Mainly for cases with larger mitochondrial genomes and more repetitive regions and complex structures, resulting in poor sequencing coverage, which cannot be looped using only second-generation random sequencing and cannot be Gap-completed using first-generation sequencing.

b) No high-quality mitochondrial reference genome, for the case where the reference genome of closely related species cannot be looped.

Mollusk Mitochondrial Sequencing Services

• Standard analysis

1. Description of raw sequencing data.

2. Raw sequencing data quality control.

3. Raw sequencing data quality cut and statistics.

4. Genome assembly.

5. Gene prediction.

6. rRNA/tRNA search.

7. Gene function annotation.

8. Genome circle mapping.

• Advanced analysis

1. Genome-wide covariance analysis.

2. SNP, Indel detection, and annotation.

3. SV detection.

4. Phylogenetic analysis of samples.

5. SSR marker development based on sequence sequencing results.

• Technical parameters

• Sequencing solution

Sequencing of Total DNA samples.

• Sample requirements

Type: non-degraded, non-contaminated DNA.

Total amount: >1 μg.

Concentration: >20 ng/μL.

• Features

The effective rate is greatly affected by sample genome size and organelle copy number, high randomness.

• Applicable objects

Suitable for reference sequences with mitochondria of closely related species.

Technical advantages

• Flexible experimental protocols

Customers can adopt suitable sequencing protocols according to different needs; strict experimental flow from DNA extraction and quality control to library construction and up-sequencing, thus ensuring high accuracy of data. Strong analysis team; strong bioinformatics team, providing personalized bioinformatics services in addition to standard bioinformatics services.

• Experienced team

Have completed mitochondrial/follow-up sequencing for a variety of species.

• Personalized analysis

We have rich experience in personalized analysis and can choose the most suitable analysis software according to the project’s needs, only to guarantee the most accurate results.

• Accurate data

Strict quality control processes guarantee the accuracy of results.

If you are interested in our services, or if the service you want is not listed, please feel free to contact us, and we will get back to you as soon as possible.